Experiment information
Accession CRX238967
Organism Mus musculus
Title Nude_Foxn1nu_4
BioProject PRJCA004965
BioSample SAMC354706
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
mMCE sgRNA library was transduced into MC38 cells for tumorigenesis in nude or C57 mice with different treatment to validate profiled tumor heterogeneity. Together with inoculation, cultured MC38 cells with mMCE library were collected as in vitro baseline. Tumors were randomly divided into the following groups, nude mice or WT mice with IgG, PD1, PDL1 or CD4 and CD8 antibodies treatment. Before treatment, five additional WT tumors were collected as in vivo baseline. Nine days after treatment, tumors were collected and grinded into homogeneous powders in liquid nitrogen. Genomic DNA were extracted from the powders. AMPLICON OTHER PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-09-15
Run
Run accession Run data file information
File nameFile size (MB)
CRR282318 CRR282318_f1.fastq.gz
CRR282318_r2.fastq.gz
472.38
552.24
SubmitterTian-Yu Song (songtianyu@zju.edu.cn)
OrganizationShanghaiTech University
Date submitted2021-05-10