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mMCE sgRNA library was transduced into MC38 cells for tumorigenesis in nude or C57 mice with different treatment to validate profiled tumor heterogeneity. Together with inoculation, cultured MC38 cells with mMCE library were collected as in vitro baseline. Tumors were randomly divided into the following groups, nude mice or WT mice with IgG, PD1, PDL1 or CD4 and CD8 antibodies treatment. Before treatment, five additional WT tumors were collected as in vivo baseline. Nine days after treatment, tumors were collected and grinded into homogeneous powders in liquid nitrogen. Genomic DNA were extracted from the powders. |
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