| Accession | CRX240130 |
| Organism | Mus musculus |
| Title | GFP-negative_rep2 |
| BioProject | PRJCA004978 |
| BioSample | SAMC355735 |
| Platform | Illumina HiSeq 2000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
For RNA-seq,total RNA was subjected to two rounds of purification using poly-T oligo-attached magnetic beads before the synthesis of double-stranded (ds) cDNA. The ds-cDNA was ligated to adaptors and sequenced with illumina Hiseq platform. For low-input KLF3-GFP positive and negative cells, Samrt-seq2 protocol was applied to construct RNA libraries. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| Release date | 2022-01-01 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR283493 |
CRR283493_f1.fastq.gz
CRR283493_r2.fastq.gz
|
1,895.09
2,323.39
|
|
| Submitter | Yangming Wang (yangming.wang@pku.edu.cn) |
|---|
| Organization | Peking University |
|---|
| Date submitted | 2021-05-14 |
|---|
