| Accession | CRX240616 |
| Organism | Mus musculus |
| Title | RFPHigh-Repl.2 |
| BioProject | PRJCA004978 |
| BioSample | SAMC373650 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Examination of differential expressed genes between sorted RFP low and RFP high mouse embryonic stem cells by poly(A)+ RNA-Seq. Cells were treated with TRIzol and followed with phase seperation, RNA precipitation and RNA wash. Finally, RNA were dissolved in DEPC water.Library was constructed with NEBNext® Ultra™ Directional RNA Library Prep Kit. Briefly, total RNA was subjected to two rounds of purification using poly-T oligo-attached magnetic beads before the synthesis of double-stranded (ds) cDNA. The ds cDNA was ligated to adaptors and sequenced with illumina HiseqXten platform. Two independently prepared mouse ESCs samples were used for RNA-seq. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2021-06-25 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR284002 |
CRR284002_f1.fastq.gz
CRR284002_r2.fastq.gz
|
770.49
846.9
|
|
| Submitter | Yangming Wang (yangming.wang@pku.edu.cn) |
|---|
| Organization | Peking University |
|---|
| Date submitted | 2021-05-19 |
|---|
