| Accession | CRX247967 |
| Organism | Mus musculus |
| Title | I_PN5_em19_1 |
| BioProject | PRJCA005396 |
| BioSample | SAMC392074 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
For DNA part, the magnetic beads containing cell nuclei (DNA) were treated with EZ-96 DNA Methylation-Direct MagPrep kit (Zymo, Cat. D5044) to complete the bisulfite conversion. Then four rounds of amplification were conducted by Klenow exo- (ENzymics, Cat. NG202) and scBS-seq-P5-N6-oligo1 (CTACACGACGCTCTTCCGATCTNNNNNN). The purified products were used to perform the second strand synthetizations using scBS-seq-P7-N6-oligo2 (AGACGTGTGCTCTTCCGATCTNNNNN). Finally, the DNA library were constructed with incorporated universal primers and index primers (New England Biolabs). After purification twice with 0.8×AMPure XP beads the DNA libraries were checked for quality and each cell was sequenced for about 3Gb on Illumina Hiseq Xten platform (Novogene). |
Bisulfite-Seq |
GENOMIC SINGLE CELL |
other |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2022-06-28 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR291550 |
CRR291550_f1.fastq.gz
CRR291550_r2.fastq.gz
|
738.08
739.25
|
|
| Submitter | Xiaoyang Zhao (zhaoxiaoyang@smu.edu.cn) |
|---|
| Organization | Southern Medical University |
|---|
| Date submitted | 2021-06-13 |
|---|
