logo
Experiment information
Accession CRX248004
Organism Mus musculus
Title R_2C_em6_1
BioProject PRJCA005396
BioSample SAMC392111
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
For DNA part, the magnetic beads containing cell nuclei (DNA) were treated with EZ-96 DNA Methylation-Direct MagPrep kit (Zymo, Cat. D5044) to complete the bisulfite conversion. Then four rounds of amplification were conducted by Klenow exo- (ENzymics, Cat. NG202) and scBS-seq-P5-N6-oligo1 (CTACACGACGCTCTTCCGATCTNNNNNN). The purified products were used to perform the second strand synthetizations using scBS-seq-P7-N6-oligo2 (AGACGTGTGCTCTTCCGATCTNNNNN). Finally, the DNA library were constructed with incorporated universal primers and index primers (New England Biolabs). After purification twice with 0.8×AMPure XP beads the DNA libraries were checked for quality and each cell was sequenced for about 3Gb on Illumina Hiseq Xten platform (Novogene). Bisulfite-Seq GENOMIC SINGLE CELL other PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-06-28
Run
Run accession Run data file information
File nameFile size (MB)
CRR291587 CRR291587_f1.fastq.gz
CRR291587_r2.fastq.gz
736.43
746.49
SubmitterXiaoyang Zhao (zhaoxiaoyang@smu.edu.cn)
OrganizationSouthern Medical University
Date submitted2021-06-13
Related experiments
Experiments(480)