| 实验编号 | CRX248246 |
| 物种名称 | Mus musculus |
| 标题 | spermatid_8 |
| 项目编号 | PRJCA005396 |
| 样本编号 | SAMC392353 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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For DNA part, the magnetic beads containing cell nuclei (DNA) were treated with EZ-96 DNA Methylation-Direct MagPrep kit (Zymo, Cat. D5044) to complete the bisulfite conversion. Then four rounds of amplification were conducted by Klenow exo- (ENzymics, Cat. NG202) and scBS-seq-P5-N6-oligo1 (CTACACGACGCTCTTCCGATCTNNNNNN). The purified products were used to perform the second strand synthetizations using scBS-seq-P7-N6-oligo2 (AGACGTGTGCTCTTCCGATCTNNNNN). Finally, the DNA library were constructed with incorporated universal primers and index primers (New England Biolabs). After purification twice with 0.8×AMPure XP beads the DNA libraries were checked for quality and each cell was sequenced for about 3Gb on Illumina Hiseq Xten platform (Novogene). |
Bisulfite-Seq |
GENOMIC SINGLE CELL |
other |
PAIRED
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| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2022-06-28 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR291829 |
CRR291829_f1.fastq.gz
CRR291829_r2.fastq.gz
|
748.02
779.86
|
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| 提交者 | Xiaoyang Zhao (zhaoxiaoyang@smu.edu.cn) |
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| 所属单位 | Southern Medical University |
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| 提交日期 | 2021-06-13 |
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