| 实验编号 | CRX250830 |
| 物种名称 | Mus musculus |
| 标题 | K9ac-zygote-rep1 |
| 项目编号 | PRJCA005419 |
| 样本编号 | SAMC394750 |
| 测序平台 | Illumina NovaSeq 5000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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For ULI-NChIP-seq, 300-500 blastomeres or 5 x 10^6 sperm were used per reaction, and two replicates were performed for each stage of wild-type embryos and one replicate for SCNT embryos. All isolated blastomeres were washed three times in 0.5% BSA in DPBS solution to avoid possible contamination. The ULI-NChIP procedure was performed as previously described (Gao et al., 2018; Liu et al., 2016b). One microgram of histone H3K9ac or H3K9me3 antibody was used for each immunoprecipitation reaction. The sequence libraries were generated using the KAPA HyperPrep Kit for the Illumina platform (kk8504), following the manufacturer’s instructions. Paired-end 150 bp sequencing was performed on NovaSeq (Illumina) platform at the Berry Genomics Co.,
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ChIP-Seq |
GENOMIC |
ChIP |
PAIRED
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| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2021-06-25 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
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| CRR294528 |
CRR294528_f1.fq.gz
CRR294528_r2.fq.gz
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3,495.11
3,920.23
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| 提交者 | Hongjie Yao (yao_hongjie@gibh.ac.cn) |
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| 所属单位 | Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences |
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| 提交日期 | 2021-06-23 |
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