logo
Experiment information
Accession CRX263270
Organism Arabidopsis thaliana
Title myc4ck3
BioProject PRJCA005944
BioSample SAMC439548
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
For transcriptome sequencing, the second pair of true leaves of the 18-day wild-type (WT) (Col-0) and myc-related mutants (myc2-2, myc3, myc4, myc2/3, myc2/4, myc3/4, and mycT) were wounded and harvested 4 h post wounding. The corresponding untreated plants were used as controls (CK). Each sample contained three biological replicates. Total RNA was extracted and approximately 1 ug of total RNA was used to enrich poly(A) mRNA using oligo-dT magnetic beads (Invitrogen, Waltham, MA, United States), followed by fragmentation into 100-400 nt sizes; and the fragments were subsequently used to synthesize cDNA with random hexamer primers (Invitrogen, Waltham, MA, United States). RNA sequencing was performed on an Illumina HiSeqXten platform (Illumina, San Diego, CA, United States) at Majorbio (Shanghai, China). RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-08-23
Run
Run accession Run data file information
File nameFile size (MB)
CRR309262 CRR309262_f1.fastq.gz
CRR309262_r2.fastq.gz
2,017.78
2,057.95
SubmitterZhi John Lu (lulab1@mail.tsinghua.edu.cn)
OrganizationTSINGHUA UNIVERSITY
Date submitted2021-08-05