| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
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For transcriptome sequencing, the second pair of true leaves of the 18-day wild-type (WT) (Col-0) and myc-related mutants (myc2-2, myc3, myc4, myc2/3, myc2/4, myc3/4, and mycT) were wounded and harvested 4 h post wounding. The corresponding untreated plants were used as controls (CK). Each sample contained three biological replicates. Total RNA was extracted and approximately 1 ug of total RNA was used to enrich poly(A) mRNA using oligo-dT magnetic beads (Invitrogen, Waltham, MA, United States), followed by fragmentation into 100-400 nt sizes; and the fragments were subsequently used to synthesize cDNA with random hexamer primers (Invitrogen, Waltham, MA, United States). RNA sequencing was performed on an Illumina HiSeqXten platform (Illumina, San Diego, CA, United States) at Majorbio (Shanghai, China). |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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