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Experiment information
Accession CRX265235
Organism Arabidopsis thaliana
Title Col-0-GFP-rep2_CHIP-seq_tlm2
BioProject PRJCA005944
BioSample SAMC442715
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
A 2-g quantity of 10-day-old Arabidopsis seedlings was ground into powder and cross-linked with 1% formaldehyde (Sigma, F8775) in 15 ml of lysis buffer (20 mM Tris-HCl, pH 7.5, 20 mM KCl, 2 mM EDTA, pH 8.0, 2.5 mM MgCl2, 25% glycerol, 250 mM sucrose, 5 mM DTT, 0.1 mM PMSF, and Roche protease inhibitor cocktail) for 20 min at 4 °C. libraries were prepared for ChIP-seq ChIP-Seq GENOMIC ChIP PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Planned read length (bp): 50
Release date2021-08-23
Run
Run accession Run data file information
File nameFile size (MB)
CRR311348 CRR311348_f1.fq.gz
CRR311348_r2.fq.gz 		2,455.16
2,637.19
SubmitterZhi John Lu (lulab1@mail.tsinghua.edu.cn)
OrganizationTSINGHUA UNIVERSITY
Date submitted2021-08-11
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