| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Libraries for GRID-seq were constructed as previously described (Li et al, 2017) with minor modifications. Briefly, after end repair and adenylation of the 3’ ends, the 84-bp DNA fragments were ligated with adaptors using T4 DNA ligase (NEB, M0202M). After PCR amplification, the 210-bp products were purified by using AMPure XP beads. Libraries were paired-end sequenced on an Illumina HiSeqX-Ten platform. |
OTHER |
OTHER |
PCR |
PAIRED
|
|
