| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Paired-end bisulfite sequencing libraries were generated using the NEXTflex Bisulfite-Seq kit (BIOO SCIENTIFIC, NOVA-511911). Briefly, genomic DNA was sonicated to ~300 bp, end repaired and 3’ adenylated. The products were ligated with methylated adapters and then subject to size selection using Ampure XP purification beads (Beckman, A63881). Bisulfite treatment was carried out using the EZ DNA Methylation-Gold kit (ZYMO Research, D5005) according to the manufacturer’s instructions. Bisulfite-converted DNA products were amplified by PCR for 15 cycles using EX Taq DNA polymerase (Takara, RR001C). The PCR products were cleaned up using Ampure XP purification beads (Beckman, A63881). The constructed libraries were validated using the Agilent 2100 Bioanalyzer and paired-end sequenced in 150-bp on an Illumina X-Ten platform. |
Bisulfite-Seq |
OTHER |
RANDOM PCR |
PAIRED
|
|
