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Experiment information
Accession CRX265368
Organism Oryza sativa
Title WT_base_BSseq180209_rep3
BioProject PRJCA005944
BioSample SAMC442848
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Paired-end bisulfite sequencing libraries were generated using the NEXTflex Bisulfite-Seq kit (BIOO SCIENTIFIC, NOVA-511911). Briefly, genomic DNA was sonicated to ~300 bp, end repaired and 3’ adenylated. The products were ligated with methylated adapters and then subject to size selection using Ampure XP purification beads (Beckman, A63881). Bisulfite treatment was carried out using the EZ DNA Methylation-Gold kit (ZYMO Research, D5005) according to the manufacturer’s instructions. Bisulfite-converted DNA products were amplified by PCR for 15 cycles using EX Taq DNA polymerase (Takara, RR001C). The PCR products were cleaned up using Ampure XP purification beads (Beckman, A63881). The constructed libraries were validated using the Agilent 2100 Bioanalyzer and paired-end sequenced in 150-bp on an Illumina X-Ten platform. Bisulfite-Seq OTHER RANDOM PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Planned read length (bp): 75
Release date2024-04-16
Run
Run accession Run data file information
File nameFile size (MB)
CRR311481 CRR311481_f1.fq.gz
CRR311481_r2.fq.gz
5,238.96
5,497.11
SubmitterZhi John Lu (lulab1@mail.tsinghua.edu.cn)
OrganizationTSINGHUA UNIVERSITY
Date submitted2021-08-11