| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Libraries for sRNA-seq were constructed as previously described (Yang et al, 2016) with minor modifications. Briefly, 3'adaptor, DNA oligonucleotide with 5'adenylation, was obtained using 5' DNA Adenylation Kit (NEB, E2601L). Total RNA was ligated to 3 adaptor using T4 RNA ligase 2, truncated KQ (NEB, M0373L). Following annealing with reverse transcription primer, RNA was ligated to 5'RNA adaptor using T4 RNA ligase 1 (NEB, M0204L). After reverse transcription and PCR amplification, 135-160-bp products were gel-purified. Libraries were pair-end sequenced on an Illumina HiSeqX-Ten platform. |
ncRNA-Seq |
OTHER |
size fractionation |
PAIRED
|
|
