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Experiment information
Accession CRX265403
Organism Oryza sativa
Title 3037_prophase_I_sRNA_rep2
BioProject PRJCA005944
BioSample SAMC442883
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Libraries for sRNA-seq were constructed as previously described (Yang et al, 2016) with minor modifications. Briefly, 3'adaptor, DNA oligonucleotide with 5'adenylation, was obtained using 5' DNA Adenylation Kit (NEB, E2601L). Total RNA was ligated to 3 adaptor using T4 RNA ligase 2, truncated KQ (NEB, M0373L). Following annealing with reverse transcription primer, RNA was ligated to 5'RNA adaptor using T4 RNA ligase 1 (NEB, M0204L). After reverse transcription and PCR amplification, 135-160-bp products were gel-purified. Libraries were pair-end sequenced on an Illumina HiSeqX-Ten platform. ncRNA-Seq OTHER size fractionation PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Planned read length (bp): 50
Release date2024-04-16
Run
Run accession Run data file information
File nameFile size (MB)
CRR311516 CRR311516_f1.fq.gz
CRR311516_r2.fq.gz
1,050.4
1,130.43
SubmitterZhi John Lu (lulab1@mail.tsinghua.edu.cn)
OrganizationTSINGHUA UNIVERSITY
Date submitted2021-08-11