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Experiment information
Accession CRX265420
Organism Oryza sativa
Title mel1_prophase_I_RNA_rep1
BioProject PRJCA005944
BioSample SAMC442900
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Libraries for mRNA-seq were constructed using the Smart2-seq method40. Briefly, total RNA was used for reverse transcription and cDNA second strand synthesis using SuperScript II reverse transcriptase (Invitrogen,18064-14) and template-switching oligos. After PCR preamplification and purification with AMPure XP beads (Beckman, A63880), the products were subjected to fragmentation and adaptor ligation using TruePrepTM DNA Library Prep Kit (Vazyme, TD503). PCR amplification was then performed with index-containing primers and 300-800-bp products were purified with AMPure XP beads. Libraries were pair-end sequenced on an Illumina HiSeqX-Ten platform by Annoroad Gene Technology (Beijing). RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Planned read length (bp): 50
Release date2024-04-16
Run
Run accession Run data file information
File nameFile size (MB)
CRR311533 CRR311533_f1.fq.gz
CRR311533_r2.fq.gz
1,630.77
1,712.24
SubmitterZhi John Lu (lulab1@mail.tsinghua.edu.cn)
OrganizationTSINGHUA UNIVERSITY
Date submitted2021-08-11