| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
To enrich the mRNA, two rounds of hybridization to oligo (dT) beads were performed on 7 μg total RNA of each sample. Ribosomal RNA contamination was analyzed using an RNA picochip with a BioAnalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). The mRNA generated was used to establish cDNA libraries using an RNA-seq sample preparation kit (Illumina, San Diego, CA, USA). The cDNA libraries were sequenced separately using an Illumina HiSeq 2500 with a 100-bp pair-end read length. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
