| Accession | CRX269623 |
| Organism | mouse gut metagenome |
| Title | 16S rRNA sequencing of mice cecal A7 |
| BioProject | PRJCA006283 |
| BioSample | SAMC448969 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The V3-V4 region of the bacterial 16S rRNA gene was amplified with the common primer pair combined with adapter sequences and barcode sequences. PCR amplification was performed in a total volume of 50 μl, which contained 60 ng genome DNA. The PCR products from the first step PCR were purified through VAHTSTM DNA Clean Beads. A second round PCR was then performed in a 40μl reaction which contained 10μl PCR products. PCR products were quantified by Quant-iT™ dsDNA HS Reagentand pooled together. |
WGS |
METAGENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
|
| Release date | 2021-08-26 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR316127 |
CRR316127_f1.fq.gz
CRR316127_r2.fq.gz
|
3.55
3.8
|
|
| Submitter | Biqing Chen (chenbiqing333@163.com) |
|---|
| Organization | Jiangsu Province Hospital of Chinese Medicine |
|---|
| Date submitted | 2021-08-25 |
|---|
