Experiment information
Accession CRX272113
Organism Severe acute respiratory syndrome coronavirus 2
Title h7.5.1-14-3
BioProject PRJCA003618
BioSample SAMC453265
Platform MGISEQ-2000RS
Library
Library name Construction protocol Strategy Source Selection Layout
RNA were extracted from infected cells using TRIzol reagent (Invitrogen) and were further enriched for mRNA using Dynabeads mRNA Purification Kit (Invitrogen, Cat. No. 61006), followed by fragmentation, reverse transcription, and double-stranded cDNA synthesis. We ligated adaptor at the end of the repaired double-stranded cDNA and cyclized the PCR products using MGIEasy mRNA Library Prep Kit (no. 85-05536-01, MGI Technology, Shenzhen, China), the libraries were loaded into the chip of the MGI2000 platform for paired-ends sequencing based on DNA nano ball (DNB) technology, with a read length of 150 bp. RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-09-13
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR318848 2021-09-13 H14-3_clean_1.fq.gz
H14-3_clean_2.fq.gz
3,362.99
3,704.18
SubmitterLijia Jia (jialj@wh.iov.cn)
OrganizationWuhan institute of virology
Date submitted2021-09-03