Experiment information
Accession CRX282135
Organism Mus musculus
Title Control_2
BioProject PRJCA006519
BioSample SAMC465119
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
The RNA sequencing was performed by Novogene Co. Ltd (Beijing, China). Total RNAs from specimens were extracted using TRIzol reagent (Invitrogen, Carsbad, CA). The concentration of extracted RNA was measured using the Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). The integrity of RNA was determined with an RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then, sequencing libraries were generated using the NEBNext® UltraTM RNA library Prep Kit for Illumina® (NEB, USA). The library preparations were sequenced on an Illumina Hiseq platform. Raw data was firstly processed using in-house perl scripts. RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2021-10-11
Run
Run accession Run data file information
File nameFile size (MB)
CRR329753 CRR329753_f1.fq.gz
CRR329753_r2.fq.gz
1,496.61
1,520.7
SubmitterTaotao Han (hantao.t@163.com)
OrganizationPeking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences
Date submitted2021-10-11