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Experiment information
Accession CRX284031
Organism Acanthopagrus schlegelii
Title the Gut Microbiota of the Juvenile Black Sea Bream (Acanthopagrus schlegelii) 6
BioProject PRJCA006770
BioSample SAMC463599
Platform Illumina NovaSeq 5000
Library
Library name Construction protocol Strategy Source Selection Layout
total genomic DNA was extracted using the bacterial DNA extraction kits (Tiangen, China) as manufacturer’s instructions. Extracted DNA was quantified by NanoDrop ND-2000 spetrophotometer (Thermo scientific, Waltham, USA) and then visualized on 1.0% agarose gels for quality and integrity verification, DNA extracts were stored at -20°C until further analysis were performed. For each extract, a fragment of 16S rRNA V3-V4 region was amplified using primer pairs of 338F and 806R. The processed PCR products were used for paired-end sequencing. High-throughput sequencing was performed using an Illumina Novaseq platform. AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 420
Planned read length (bp) for mate 2: 420
Release date2021-10-21
Run
Run accession Run data file information
File nameFile size (MB)
CRR331724 CRR331724_f1.fq.gz
CRR331724_r2.fq.gz
5.72
6.97
SubmitterPeng Sun (sunpeng0512@hotmail.com)
OrganizationEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
Date submitted2021-10-21