| Accession | CRX284035 |
| Organism | Acanthopagrus schlegelii |
| Title | the Gut Microbiota of the Juvenile Black Sea Bream (Acanthopagrus schlegelii) 10 |
| BioProject | PRJCA006770 |
| BioSample | SAMC463603 |
| Platform | Illumina NovaSeq 5000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
total genomic DNA was extracted using the bacterial DNA extraction kits (Tiangen, China) as manufacturer’s instructions. Extracted DNA was quantified by NanoDrop ND-2000 spetrophotometer (Thermo scientific, Waltham, USA) and then visualized on 1.0% agarose gels for quality and integrity verification, DNA extracts were stored at -20°C until further analysis were performed. For each extract, a fragment of 16S rRNA V3-V4 region was amplified using primer pairs of 338F and 806R. The processed PCR products were used for paired-end sequencing. High-throughput sequencing was performed using an Illumina Novaseq platform. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 420
Planned read length (bp) for mate 2: 420
|
| Release date | 2021-10-21 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR331728 |
CRR331728_f1.fq.gz
CRR331728_r2.fq.gz
|
6.03
7
|
|
| Submitter | Peng Sun (sunpeng0512@hotmail.com) |
|---|
| Organization | East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
|---|
| Date submitted | 2021-10-21 |
|---|
