| 实验编号 | CRX284041 |
| 物种名称 | Acanthopagrus schlegelii |
| 标题 | the Gut Microbiota of the Juvenile Black Sea Bream (Acanthopagrus schlegelii) 16 |
| 项目编号 | PRJCA006770 |
| 样本编号 | SAMC463609 |
| 测序平台 | Illumina NovaSeq 5000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
total genomic DNA was extracted using the bacterial DNA extraction kits (Tiangen, China) as manufacturer’s instructions. Extracted DNA was quantified by NanoDrop ND-2000 spetrophotometer (Thermo scientific, Waltham, USA) and then visualized on 1.0% agarose gels for quality and integrity verification, DNA extracts were stored at -20°C until further analysis were performed. For each extract, a fragment of 16S rRNA V3-V4 region was amplified using primer pairs of 338F and 806R. The processed PCR products were used for paired-end sequencing. High-throughput sequencing was performed using an Illumina Novaseq platform. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 420
Planned read length (bp) for mate 2: 420
|
| 发布日期 | 2021-10-21 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR331734 |
CRR331734_f1.fq.gz
CRR331734_r2.fq.gz
|
6.02
7.19
|
|
| 提交者 | Peng Sun (sunpeng0512@hotmail.com) |
|---|
| 所属单位 | East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
|---|
| 提交日期 | 2021-10-21 |
|---|
