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Experiment information
Accession CRX288815
Organism Triticum aestivum
Title SPL1D_2
BioProject PRJCA007017
BioSample SAMC476009
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
Full length TF was cloned into pIX-Halo vector, the HALO-SPL protein was generated using 500 ng of pIX-HALO-SPL plasmid and the TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) according to the manufacturer’s protocol. The reaction was then incubated with 10 μL of Magne-HALO Tag beads (Promega, G7282) for 1 h at 25 °C in 1× PBS with 0.005% Nonidet P-40 (PBST). Bound protein was washed five times with PBST and subsequently treated with DNaseI. The DNA-free HALO-SPL protein was incubated with 500 μg of ultrasonicated genomic DNA library (200~800 bp) for 1 h at 25 °C. The beads were then washed eight times with PBST and incubated with 1μl Tn5 transposomes in 40 μl of tagmentation buffer (10 mM TAPS-NaOH ph 8.0, 5 mM MgCl2) at 55°C for 10 minutes. The DNA were recovered by NEB Monarch™ DNA Cleanup Kit (T1030L) and then amplified using Phusion DNA polymerase for 10-13 cycles. Amplified libraries were purified with AMPure beads to remove primers. OTHER GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2022-12-31
Run
Run accession Run data file information
File nameFile size (MB)
CRR336679 CRR336679_f1.fastq.gz
CRR336679_r2.fastq.gz
11,447.78
11,707.65
SubmitterZefu Lu (luzefu@caas.cn)
OrganizationInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences
Date submitted2021-11-02