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Experiment information
Accession CRX288833
Organism Triticum aestivum
Title SPL7A
BioProject PRJCA007017
BioSample SAMC476027
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
Full length TF was cloned into pIX-Halo vector, the HALO-SPL protein was generated using 500 ng of pIX-HALO-SPL plasmid and the TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) according to the manufacturer’s protocol. The reaction was then incubated with 10 μL of Magne-HALO Tag beads (Promega, G7282) for 1 h at 25 °C in 1× PBS with 0.005% Nonidet P-40 (PBST). Bound protein was washed five times with PBST and subsequently treated with DNaseI. The DNA-free HALO-SPL protein was incubated with 500 μg of ultrasonicated genomic DNA library (200~800 bp) for 1 h at 25 °C. The beads were then washed eight times with PBST and incubated with 1μl Tn5 transposomes in 40 μl of tagmentation buffer (10 mM TAPS-NaOH ph 8.0, 5 mM MgCl2) at 55°C for 10 minutes. The DNA were recovered by NEB Monarch™ DNA Cleanup Kit (T1030L) and then amplified using Phusion DNA polymerase for 10-18 cycles. Amplified libraries were purified with AMPure beads to remove primers. OTHER GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2022-12-31
Run
Run accession Run data file information
File nameFile size (MB)
CRR336697 CRR336697_f1.fastq.gz
CRR336697_r2.fastq.gz
5,901.08
5,334.86
SubmitterZefu Lu (luzefu@caas.cn)
OrganizationInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences
Date submitted2021-11-02