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After the RNA sample passes the test, enrich the eukaryotic mRNA with magnetic beads with Oligo (dT); add Fragmentation Buffer to randomly interrupt the mRNA; use the mRNA as a template and synthesize the first with random hexamers One cDNA strand, then add buffer, dNTPs, RNase H and DNA polymerase I to synthesize the second cDNA strand, and use AMPure XP beads to purify the cDNA; the purified double-stranded cDNA is then subjected to end repair, A tail is added and the sequencing adapter is connected, Then use AMPure XP beads to select the size of the fragments; finally enrich the cDNA library by PCR. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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