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实验基本信息
实验编号 CRX291085
物种名称 Paeonia rockii
标题 Seed-miR-F3
项目编号 PRJCA007214
样本编号 SAMC479610
测序平台 Illumina HiSeq 2500
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
First of all, ligated the 3 SR and 5 SR Adaptor. Then, reverse transcription synthetic first chain. Last, PCR amplification and Size Selection. PAGE gel was used to electrophoresis fragment screening purposes, rubber cutting recycling as the pieces get small RNA libraries. At last, PCR products were purified (AMPure XP system) and library quality was assessed. miRNA-Seq OTHER PCR SINGLE
处理信息 Planned read length (bp) for mate 1: 500
Planned read length (bp) for mate 2: 500
Planned read length (bp): 30
发布日期2022-12-31
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR339333 CRR339333.fq.gz 456.26
提交者jiarui Zheng (zhengjiarui1222@163.com)
所属单位Yangtze University
提交日期2021-11-15
关联实验
Experiments(17)