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Experiment information
Accession CRX294231
Organism Alsophila spinulosa
Title small_RNA-1
BioProject PRJCA006485
BioSample SAMC489743
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
We used magnetic beads with Oli (dT) to enrich the mRNA and broke it into pieces by applying a fragmentation buffer. Employing these mRNAs as the template, random hexamers were used to synthesize the first-strand cDNA. Next, together with buffer, the dNTPs, RNaseH, and DNA polymerase I synthesized the second-strand cDNA. Each cDNA library was purified and to it a joint end with A was added. OTHER GENOMIC PCR SINGLE
Processing Planned read length (bp): 50
Release date2022-03-18
Run
Run accession Run data file information
File nameFile size (MB)
CRR342770 CRR342770.fq.gz 238.51
Submitterxiong huang (huangxiongcaf@163.com)
OrganizationChinese Academy of Forestry
Date submitted2021-11-23
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Experiments(5)