| 实验编号 | CRX306223 |
| 物种名称 | Musa acuminata |
| 标题 | Transcriptome profiling of banana peel during its developing and ripening stages GM-1 |
| 项目编号 | PRJCA007612 |
| 样本编号 | SAMC536942 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to manufacturer’s recommendations. Index codes were added to attribute sequences to each sample. mRNA was purified from total RNA using poly-T oligo-attached mag netic beads. The first strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-) while the second one was done using DNA polymerase I and RNase H.The library preparations were sequenced on an Illumina Hiseq 2000/2500 platform and 100bp/50bp single-end reads were generated. |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 250
|
| 发布日期 | 2021-12-21 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR355579 |
CRR355579_f1.fq.gz
CRR355579_r2.fq.gz
|
4,094.65
4,219.03
|
|
| 提交者 | tong ning (1686413560@qq.com) |
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| 所属单位 | South China Agricultural University |
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| 提交日期 | 2021-12-17 |
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