| Accession | CRX307004 |
| Organism | Capra hircus |
| Title | D4501 |
| BioProject | PRJCA007633 |
| BioSample | SAMC539143 |
| Platform | Illumina HiSeq 2000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The total RNA of samples was extracted using RNeasy Plus Universal Mini Kit (QIAGEN, Germany). A total amount of 2 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using VAHTS mRNA-seq v2 Library Prep Kit for Illumina were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using fragmentation buffer. First strand cDNA was synthesized and second strand cDNA synthesis was subsequently performed. Remaining overhangs were converted into blunt ends. After adenylation of 3’ ends of DNA fragments, adaptor with hairpin loop structure were ligated. Then the PCR was performed. At last, Qubit HS quantification, Agilent 2100 Bioanalyzer/Fragment Analyzer 5300 quality control, the final library size of about 350bp. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| Release date | 2021-12-29 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR356440 |
CRR356440_f1.fastq.gz
CRR356440_r2.fastq.gz
|
2,365.6
2,956.81
|
|
| Submitter | Yue Liu (liuyue196160@163.com) |
|---|
| Organization | Institute of Animal Sciences, Chinese Academy of Agricultural Sciences |
|---|
| Date submitted | 2021-12-23 |
|---|
