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Sequencing libraries were generated using the Ion Plus Fragment Library Kit 48 rxns (Thermo Scientific, USA) according to the manufacturer's instructions. Using molecular barcodes for parallelization, these markers were amplified using specific primers (16S V4-V5: 515F-907R). All polymerase chain reaction (PCR) reactions were carried out in 30 μL reactions with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers, and about 10 ng templates DNA. |
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GENOMIC |
PCR |
SINGLE
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