Accession | CRX316657 |
Organism | sheep gut metagenome |
Title | Wi45 |
BioProject | PRJCA007872 |
BioSample | SAMC553560 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Using TIANamp Stool DNA Kit extraction sheep feces microbial genome DNA, The V3-V4 region of 16S rRNA gene was selected as the sequencing region, The specific primers (338F-806R) with a barcode are then synthesized, and the amplification was using the TransStart Fastpfu DNA Polymerase system. Then the HiSeq libraries were constructed. Illumina HiSeq 2500 platform was used for sequencing and the sequencing read length was PE250. |
AMPLICON |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 468
|
Release date | 2022-01-14 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR368404 |
CRR368404_f1.fq.gz
CRR368404_r2.fq.gz
|
8.49
9.05
|
|
Submitter | Xingxing Zhang (shzuzx@163.com) |
Organization | Institute of Animal Husbandry and Veterinary Medicine, Xinjiang Academy of Agricultural and Reclamation Sciences |
Date submitted | 2022-01-14 |