| Accession | CRX322578 |
| Organism | Macaca fascicularis |
| Title | H-OM2_IP |
| BioProject | PRJCA008042 |
| BioSample | SAMC559724 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
mRNA of cells form tissues was isolated by Oligo-dT beads and fragmented into 100 to 200 nt. After that, 1 ug fragmented RNA was subjected to the MeRIP procedure to enrich methylated RNA using anti m6A antibody. After immunoprecipitation, the enriched RNA was converted to cDNA by reverse transcription.Then Illumina adapters were ligated to DNA, and amplified by PCR. |
RIP-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 200
|
| Release date | 2023-02-12 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR375423 |
CRR375423.bam
|
4,306.48
|
|
| Submitter | Weimin ci (ciwm@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2022-01-24 |
|---|
