| 实验编号 | CRX322646 |
| 物种名称 | Macaca fascicularis |
| 标题 | L-YF3-IP |
| 项目编号 | PRJCA008042 |
| 样本编号 | SAMC559758 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
mRNA of cells form tissues was isolated by Oligo-dT beads and fragmented into 100 to 200 nt. After that, 1 ug fragmented RNA was subjected to the MeRIP procedure to enrich methylated RNA using anti m6A antibody. After immunoprecipitation, the enriched RNA was converted to cDNA by reverse transcription.Then Illumina adapters were ligated to DNA, and amplified by PCR. |
RIP-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 200
|
| 发布日期 | 2023-02-12 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR375491 |
CRR375491.bam
|
5,979.12
|
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| 提交者 | Weimin ci (ciwm@big.ac.cn) |
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| 所属单位 | Beijing Institute of Genomics, Chinese Academy of Sciences |
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| 提交日期 | 2022-01-24 |
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