| 实验编号 | CRX327543 |
| 物种名称 | Severe acute respiratory syndrome coronavirus 2 |
| 标题 | RJ-LNN04155 |
| 项目编号 | PRJCA007589 |
| 样本编号 | SAMC586114 |
| 测序平台 | Illumina Nextseq 500 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
The viral RNA was extracted with MagMAX Viral / Pathogen Nucleic Acid Isolation kit and KingFisher automatic platform. Annealing of cDNA was conducted with 8,5 µl of the viral RNA extracted. Next-generation sequencing steps including library preparation were performed with Illumina COVIDSeq Test (Illumina), according to the manufacturer’s protocol. Purification was then conducted using 5 µl of each library combined, and the TapeStation (Agilent) system was used for quality control. NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles) was used in the NextSeq (Illumina). |
AMPLICON |
VIRAL RNA |
RT-PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| 发布日期 | 2022-02-05 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR380472 |
CRR380472_f1.fastq.gz
CRR380472_r2.fastq.gz
|
30.82
31.22
|
|
| 提交者 | LUIZ GONZAGA PAULA DE ALMEIDA (lgonzaga@lncc.br) |
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| 所属单位 | National Laboratory of Scientific Computation LNCC/MCTIC |
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| 提交日期 | 2022-02-04 |
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