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实验基本信息
实验编号 CRX382000
物种名称 Mus musculus
标题 Sph_D3_Venus-_84
项目编号 PRJCA008671
样本编号 SAMC683082
测序平台 MGISEQ-2000RS
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Smart-seq2 libraries were generated described as mentioned with minor optimization (Picelli, S. et al. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc 9, 171-181, doi:10.1038/nprot.2014.006 (2014)). Maxima H Minus reverse transcriptase (3U/reaction, Thermo Fisher Science, EP0751) was used for the cDNA synthesis. The prepared libraries were sequenced on using an MGISEQ2000 (MGI Tech) instrument in PE100 mode. RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
发布日期2023-10-20
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR437819 CRR437819_f1.fq.gz
CRR437819_r2.fq.gz 		144.94
125.24
CRR437820 CRR437820_f1.fq.gz
CRR437820_r2.fq.gz 		142
122.85
CRR437821 CRR437821_f1.fq.gz
CRR437821_r2.fq.gz 		143.6
123.96
CRR437822 CRR437822_f1.fq.gz
CRR437822_r2.fq.gz 		140.78
121.75
提交者Jose Silva (sciencedb@gibh.ac.cn)
所属单位Guangzhou National Laboratory
提交日期2022-03-16
关联实验
Experiments(1734)