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实验基本信息
实验编号 CRX382003
物种名称 Mus musculus
标题 Sph_D3_Venus-_87
项目编号 PRJCA008671
样本编号 SAMC683085
测序平台 MGISEQ-2000RS
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Smart-seq2 libraries were generated described as mentioned with minor optimization (Picelli, S. et al. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc 9, 171-181, doi:10.1038/nprot.2014.006 (2014)). Maxima H Minus reverse transcriptase (3U/reaction, Thermo Fisher Science, EP0751) was used for the cDNA synthesis. The prepared libraries were sequenced on using an MGISEQ2000 (MGI Tech) instrument in PE100 mode. RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
发布日期2023-10-20
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR437831 CRR437831_f1.fq.gz
CRR437831_r2.fq.gz 		111.45
112.85
CRR437832 CRR437832_f1.fq.gz
CRR437832_r2.fq.gz 		108.73
109.41
CRR437833 CRR437833_f1.fq.gz
CRR437833_r2.fq.gz 		110.22
110.89
CRR437834 CRR437834_f1.fq.gz
CRR437834_r2.fq.gz 		108.24
109.38
提交者Jose Silva (sciencedb@gibh.ac.cn)
所属单位Guangzhou National Laboratory
提交日期2022-03-16
关联实验
Experiments(1734)