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实验基本信息
实验编号 CRX760516
物种名称 Mus musculus
标题 iGata6HeterogeneousInduction_24h_39
项目编号 PRJCA008671
样本编号 SAMC2980253
测序平台 MGISEQ-2000RS
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Smart-seq2 libraries were generated described as mentioned with minor optimization (Picelli, S. et al. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc 9, 171-181, doi:10.1038/nprot.2014.006 (2014)). Maxima H Minus reverse transcriptase (3U/reaction, Thermo Fisher Science, EP0751) was used for the cDNA synthesis. The prepared libraries were sequenced on using an MGISEQ2000 (MGI Tech) instrument in PE279 mode. RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
发布日期2023-10-20
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR844021 CRR844021_f1.fq.gz
CRR844021_r2.fq.gz 		106.7
103.08
提交者Jose Silva (sciencedb@gibh.ac.cn)
所属单位Guangzhou National Laboratory
提交日期2023-08-08
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