| Accession | CRX386340 |
| Organism | Morus notabilis |
| Title | Male_CHS_flower_methylation-1 |
| BioProject | PRJCA008608 |
| BioSample | SAMC690748 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Adjust the volume of the combined gDNA sample and unmethylated lambda DNA control to a total volume of 80 μl using 1x TE and fragment the DNA to 300 bp. Blunt-ended fragments are created by filling in or chewing back 3’ and 5’ overhangs. Phosphorylation of the 5’ ends ensures the fragments are suitable for ligation. This step is dA-tailing of the 5’end of the end-repaired, phosphorylated fragments. This single A overhang enables ligation to adaptors with single T overhangs. Methylated adaptor containing sequences required downstream in the sequencing workflow are ligated to the dA-tailed fragment. |
Bisulfite-Seq |
GENOMIC |
unspecified |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
| Release date | 2022-03-28 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR443594 |
CRR443594_f1.fq.gz
CRR443594_r2.fq.gz
|
2,603.08
2,634.56
|
|
| Submitter | Aichun Zhao (zhaoaichun@swu.edu.cn) |
|---|
| Organization | Southwest University |
|---|
| Date submitted | 2022-03-21 |
|---|
