Accession | CRX386341 |
Organism | Morus notabilis |
Title | Male_CHS_flower_methylation-2 |
BioProject | PRJCA008608 |
BioSample | SAMC690749 |
Platform | Illumina NovaSeq 6000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Adjust the volume of the combined gDNA sample and unmethylated lambda DNA control to a total volume of 80 μl using 1x TE and fragment the DNA to 300 bp. Blunt-ended fragments are created by filling in or chewing back 3’ and 5’ overhangs. Phosphorylation of the 5’ ends ensures the fragments are suitable for ligation. This step is dA-tailing of the 3’end of the end-repaired, phosphorylated fragments. This single A overhang enables ligation to adaptors with single T overhangs. Methylated adaptor containing sequences required downstream in the sequencing workflow are ligated to the dA-tailed fragment. |
Bisulfite-Seq |
GENOMIC |
unspecified |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
Release date | 2022-03-28 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR443595 |
CRR443595_f1.fq.gz
CRR443595_r2.fq.gz
|
2,738.74
2,651.64
|
|
Submitter | Aichun Zhao (zhaoaichun@swu.edu.cn) |
Organization | Southwest University |
Date submitted | 2022-03-21 |