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Twenty milliliters of spore suspension (1 x 106 spores/ml) of the wild-type 70-15, the vrf1-deletion mutant, the hox7-deletion mutant, or the hox7 and vrf1 deletion mutant was inoculated on hydrophobic polyvinylchloride (PVC) films and incubated in a humidity chamber at 25 degrees Celsius for 5 h. Appressorial mRNAs in triplicate independently for each strain, were extracted and isolated using an RNeasy Plus Mini Kit (Qiagen, Germany). After RNA purification and library construction of the samples, next-generation sequencing technology (NGS) was used to perform paired-end (PE) sequencing in a 2 x 150 nt way on these libraries based on the Illumina HiSeq platform (NovaSeq 6000). The amount of data obtained by sequencing was 6 G/sample. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
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