Accession | CRX400849 |
Organism | Severe acute respiratory syndrome coronavirus 2 |
Title | RJ-LNN08322 |
BioProject | PRJCA007589 |
BioSample | SAMC716025 |
Platform | Illumina Nextseq 500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The viral RNA was extracted with MagMAX Viral / Pathogen Nucleic Acid Isolation kit and KingFisher automatic platform. Annealing of cDNA was conducted with 8,5 µl of the viral RNA extracted. Next-generation sequencing steps including library preparation were performed with Illumina COVIDSeq Test (Illumina), according to the manufacturer’s protocol. Purification was then conducted using 5 µl of each library combined, and the TapeStation (Agilent) system was used for quality control. NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles) was used in the NextSeq (Illumina). |
AMPLICON |
VIRAL RNA |
RT-PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
Release date | 2022-04-26 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR459401 |
CRR459401_f1.fastq.gz
CRR459401_r2.fastq.gz
|
32.25
33.62
|
|
Submitter | LUIZ GONZAGA PAULA DE ALMEIDA (lgonzaga@lncc.br) |
Organization | National Laboratory of Scientific Computation LNCC/MCTIC |
Date submitted | 2022-04-11 |