| Accession | CRX400899 |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Title | RJ-LNN08381 |
| BioProject | PRJCA007589 |
| BioSample | SAMC716075 |
| Platform | Illumina Nextseq 500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The viral RNA was extracted with MagMAX Viral / Pathogen Nucleic Acid Isolation kit and KingFisher automatic platform. Annealing of cDNA was conducted with 8,5 µl of the viral RNA extracted. Next-generation sequencing steps including library preparation were performed with Illumina COVIDSeq Test (Illumina), according to the manufacturer’s protocol. Purification was then conducted using 5 µl of each library combined, and the TapeStation (Agilent) system was used for quality control. NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles) was used in the NextSeq (Illumina). |
AMPLICON |
VIRAL RNA |
RT-PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| Release date | 2022-04-26 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR459451 |
CRR459451_f1.fastq.gz
CRR459451_r2.fastq.gz
|
44
45.84
|
|
| Submitter | LUIZ GONZAGA PAULA DE ALMEIDA (lgonzaga@lncc.br) |
|---|
| Organization | National Laboratory of Scientific Computation LNCC/MCTIC |
|---|
| Date submitted | 2022-04-11 |
|---|
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