Experiment information
Accession CRX400977
Organism Severe acute respiratory syndrome coronavirus 2
Title RJ-LNN08478
BioProject PRJCA007589
BioSample SAMC716153
Platform Illumina Nextseq 500
Library name Construction protocol Strategy Source Selection Layout
The viral RNA was extracted with MagMAX Viral / Pathogen Nucleic Acid Isolation kit and KingFisher automatic platform. Annealing of cDNA was conducted with 8,5 µl of the viral RNA extracted. Next-generation sequencing steps including library preparation were performed with Illumina COVIDSeq Test (Illumina), according to the manufacturer’s protocol. Purification was then conducted using 5 µl of each library combined, and the TapeStation (Agilent) system was used for quality control. NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles) was used in the NextSeq (Illumina). AMPLICON VIRAL RNA RT-PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2022-04-26
Run accession Run data file information
File nameFile size (MB)
CRR459529 LNN08478.R1.fastq.gz
SubmitterLUIZ GONZAGA PAULA DE ALMEIDA (lgonzaga@lncc.br)
OrganizationNational Laboratory of Scientific Computation LNCC/MCTIC
Date submitted2022-04-11
Related experiments