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实验基本信息
实验编号 CRX400987
物种名称 Bos taurus
标题 BHBA-4-2
项目编号 PRJCA009025
样本编号 SAMC716163
测序平台 Illumina HiSeq 2500
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Total RNA was extracted using Trizol reagent kit according to the manufacturer’s protocol. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads, while prokaryotic mRNA was enriched by removing rRNA by Ribo-ZeroTM Magnetic Kit. Then the cDNA fragments were purified with QiaQuick PCR extraction kit, end repaired, poly(A) added, and ligated to Illumina sequencing adapters. RNA-Seq TRANSCRIPTOMIC other PAIRED
处理信息 Planned read length (bp) for mate 1: 200
Planned read length (bp) for mate 2: 200
发布日期2022-06-16
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR459539 CRR459539_f1.fq.gz
CRR459539_r2.fq.gz
1,884.05
2,035.44
提交者jian fei gong (Gjf18553676192@163.com)
所属单位Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
提交日期2022-04-11