Accession | CRX416798 |
Organism | Streptococcus equi subsp. zooepidemicus |
Title | FSZ_2 |
BioProject | PRJCA009509 |
BioSample | SAMC752383 |
Platform | DNBSEQ-T7 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted by Trizol regent (each group was performed in triplicate). RNA-Seq and bioinformatics analysis were performed by Beijing Genomics Institute (BGI), China. The mRNA library was constructed and sequenced by BGISEQ-500 platform. Low quality of raw sequencing data was first filtered, then using HISAT/Bowtie2 mapping tool clean reads to reference. Gene quantification was measured by FPKM calculation according to the expectation maximization algorithm called RSEM. RNA sequencing (RNA-Seq) method was employed based on the Poisson distribution. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2022-05-11 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR476052 |
CRR476052_f1.fq.gz
CRR476052_r2.fq.gz
|
1,036.29
1,043.41
|
|
Submitter | Yao zhi yuan (932516253@qq.com) |
Organization | Jiangnan University |
Date submitted | 2022-05-10 |