Experiment information
Accession CRX416799
Organism Streptococcus equi subsp. zooepidemicus
Title FSZ_3
BioProject PRJCA009509
BioSample SAMC752384
Platform DNBSEQ-T7
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted by Trizol regent (each group was performed in triplicate). RNA-Seq and bioinformatics analysis were performed by Beijing Genomics Institute (BGI), China. The mRNA library was constructed and sequenced by BGISEQ-500 platform. Low quality of raw sequencing data was first filtered, then using HISAT/Bowtie2 mapping tool clean reads to reference. Gene quantification was measured by FPKM calculation according to the expectation maximization algorithm called RSEM. RNA sequencing (RNA-Seq) method was employed based on the Poisson distribution. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-05-11
Run
Run accession Run data file information
File nameFile size (MB)
CRR476053 CRR476053_f1.fq.gz
CRR476053_r2.fq.gz
1,031.24
1,041.37
SubmitterYao zhi yuan (932516253@qq.com)
OrganizationJiangnan University
Date submitted2022-05-10