| Accession | CRX455129 |
| Organism | Lacticaseibacillus rhamnosus |
| Title | Z3 |
| BioProject | PRJCA009980 |
| BioSample | SAMC798233 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
RNA-seq transcriptome library was prepared following TruSeq RNA sample preparation Kit from Illumina using total RNA. Shortly, ribosomal RNA depletion is performed by Ribo-Zero Magnetic kit and then all mRNAs were broken into short fragments by adding fragmentation buffer firstly. Secondly double-stranded cDNA was synthesized using a SuperScript double-stranded cDNA synthesis kit with random hexamer primers. When the second strand cDNA was synthesized, dUTP was incorporated in place of dTTP. Then the synthesized cDNA was subjected to end-repair. The second strand cDNA with dUTP was recognized and degraded by UNG enzyme. Libraries were size selected for cDNA target fragments of 200 bp on Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase for 15 PCR cycles. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2022-08-08 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR515043 |
CRR515043_f1.fastq.gz
CRR515043_r2.fastq.gz
|
691.48
689.85
|
|
| Submitter | Zhang Chenchen (werhome@126.com) |
|---|
| Organization | Yangzhou University |
|---|
| Date submitted | 2022-06-10 |
|---|
