| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was used as the starting Sample, and the small RNA 3 'end had an adapter. Reverse transcription primers were added to prevent redundant 3 ' adapters from connecting to 5' adapters and reduce the self-linking products of the adapters. Then, 19 ' adapters were added to reverse transcription and synthesize cDNA. After PCR amplification, the target DNA fragment was separated by PAGE gel electrophoresis, and the cDNA library was recovered by gel cutting, respectively, according to the Small RNA Sample Pre Kit protocol. |
RNA-Seq |
TRANSCRIPTOMIC |
RT-PCR |
SINGLE
|
|
