| 实验编号 | CRX461719 |
| 物种名称 | Pyricularia oryzae |
| 标题 | Sample 5 |
| 项目编号 | PRJCA010162 |
| 样本编号 | SAMC807041 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Two hundred microliters of spore suspensions (5 x 10,000 spores/ml) of the wild-type 70-15 or CLP1 deletion mutant were inoculated onto cellophane membranes overlaid on complete medium and incubated under a light-dark (18 h-6 h) cycle at 25 °C for 4 days. The mycelial mRNAs in triplicate independently for each strain, were extracted and isolated using an RNeasy Plus Mini Kit (Qiagen, Germany). After RNA purification and library construction of the samples, next-generation sequencing technology (NGS) was used to perform paired-end (PE) sequencing in a 2 x 150 nt way on these libraries based on the Illumina HiSeq platform (NovaSeq 6000). The amount of data obtained by sequencing was 6 G/sample. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 380
|
| 发布日期 | 2022-08-20 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR521766 |
CRR521766_f1.fastq.gz
CRR521766_r2.fastq.gz
|
1,703.2
1,772.45
|
|
| 提交者 | Jianping Lu (jplu@zju.edu.cn) |
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| 所属单位 | Zhejiang University |
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| 提交日期 | 2022-06-21 |
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