| Accession | CRX471279 |
| Organism | Mus musculus |
| Title | Ctrl3-ssRNA |
| BioProject | PRJCA010472 |
| BioSample | SAMC818769 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
1 μg total RNA was used for following library preparation. The poly(A) mRNA isolation was performed using Oligo(dT) beads. The mRNA fragmentation was performed using divalent cations and high temperature. Priming was performed using Random Primers. First strand cDNA and the second-strand cDNA were synthesized. The purified double-stranded cDNA was then treated to repair both ends and add a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. Size selection of Adaptor-ligated DNA was then performed using DNA Clean Beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated. Then libraries with different indexs were multiplexed and loaded on an llumina Novaseq6000 instrument for sequencing using a 2x150 paired-end (PE) configuration according to manufacturer’s instructions. |
RNA-Seq |
TRANSCRIPTOMIC |
RT-PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2023-07-06 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR531484 |
CRR531484_f1.fastq.gz
CRR531484_r2.fastq.gz
|
2,426.76
2,540.22
|
|
| Submitter | Wujianan Sun (swjn2017@mail.ustc.edu.cn) |
|---|
| Organization | University of Science and Technology of China |
|---|
| Date submitted | 2022-07-14 |
|---|
